Opportunity Information: Apply for G16AS00012
Apply for G16AS00012
- The DOI-USGS1 in the science and technology and other research and development sector is offering a public funding opportunity titled "Cooperative Ecosystem Studies Unit, Rocky Mountain CESU" and is now available to receive applicants.
- Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.808.
- This funding opportunity was created on Nov 18, 2015 and posted on Nov 18, 2015.
- Applicants must submit their applications by Dec 02, 2015. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
- The number of recipients for this funding is limited to 1 candidate(s).
- Eligible applicants include: Others (see text field entitled Additional Information on Eligibility for clarification).
[Watch] Creating a grant proposal using the step-by-step wizard inside the applicant portal:
Opportunity Summary:
The Cooperative Ecosystem Studies Unit, Rocky Mountain CESU funding opportunity (Funding Opportunity Number G16AS00012) is a discretionary U.S. Geological Survey (USGS) award administered under the Department of the Interior (Agency: DOI-USGS). It is offered as a cooperative agreement, and it falls under the Science and Technology and other Research and Development activity category (CFDA 15.808). The notice was posted and created on November 18, 2015, with an application closing date of December 2, 2015 (no later extension is indicated, as the current and original closing dates match).
The core purpose of this opportunity is to support specialized laboratory sequencing and bioinformatics analysis for a USGS-funded research effort focused on microbial mercury methylation in Bighorn Lake, a waterbody spanning Montana and Wyoming. The work centers on DNA that has already been extracted from sediment and water samples collected at multiple sites in the lake. In practical terms, the project is designed to characterize microbial community composition in those samples to better understand which microbes are present and how they may relate to processes that convert mercury into methylmercury, a particularly toxic form that can accumulate in aquatic food webs.
From a technical standpoint, the award supports sequencing of previously amplified PCR amplicons targeting the 16S rRNA gene, a standard marker used to profile bacteria and archaea in environmental samples. The opportunity specifies that these existing amplicons will be barcoded (indexed) so that many samples can be pooled together and run efficiently in a single sequencing run, a method commonly referred to as multiplex sequencing. The sequencing platform identified is the Illumina MiSeq, which is widely used for amplicon sequencing due to its accuracy and throughput for community profiling studies. The expected deliverable is the generation of sequencing data and associated analysis outputs that can be used by the USGS project team to interpret microbial community patterns across sampling locations in Bighorn Lake.
Eligibility is broadly listed as "Others," with the note that additional clarification would be provided in the full eligibility text field in the original posting. The notice anticipates a single award (Expected Awards: 1). No award ceiling is specified (Award Ceiling: 0), which typically indicates that the funding amount is not stated in the summary fields and would be defined in the full announcement, budget negotiations, or accompanying documentation rather than capped in the brief listing.
In short, this is a narrowly scoped research support opportunity: one cooperative agreement intended to fund the sequencing and analysis of 16S rRNA gene amplicons derived from Bighorn Lake sediment and water samples, producing microbial community data to inform a broader USGS investigation into mercury methylation in the lake ecosystem.
Frequently Asked Questions (FAQs)
What is the name of this funding opportunity?
This opportunity is the Cooperative Ecosystem Studies Unit, Rocky Mountain CESU funding opportunity.
What is the Funding Opportunity Number?
The Funding Opportunity Number is G16AS00012.
Which federal agency is offering and administering this award?
The award is a discretionary U.S. Geological Survey (USGS) award administered under the Department of the Interior (DOI), listed as DOI-USGS.
What type of award is being offered?
The opportunity is offered as a cooperative agreement.
What is the activity category for this opportunity?
The activity category is Science and Technology and other Research and Development.
What is the CFDA number associated with this opportunity?
The CFDA number is 15.808.
When was the notice posted and created?
The notice was posted and created on November 18, 2015.
When is the application closing date?
The application closing date is December 2, 2015.
Is there an extension beyond the original closing date?
No extension is indicated, because the current and original closing dates match (both are December 2, 2015).
What is the main purpose of the grant?
The purpose is to support specialized laboratory sequencing and bioinformatics analysis for a USGS-funded research effort focused on microbial mercury methylation in Bighorn Lake.
What environmental issue or process is this project trying to better understand?
The project aims to better understand microbial mercury methylation, specifically the microbial processes and community patterns that may relate to the conversion of mercury into methylmercury.
Why is methylmercury important in this context?
The opportunity notes methylmercury as a particularly toxic form of mercury that can accumulate in aquatic food webs.
Where is the study location?
The work focuses on Bighorn Lake, a waterbody that spans Montana and Wyoming.
What types of samples are involved?
The samples are sediment and water samples collected at multiple sites in Bighorn Lake.
Has DNA already been extracted from the samples?
Yes. The opportunity specifies that DNA has already been extracted from the collected sediment and water samples.
What is the project expected to characterize or measure?
The project is designed to characterize microbial community composition in the samples to determine which microbes are present and how patterns may relate to mercury methylation processes.
What specific sequencing target is identified?
The opportunity specifies sequencing of previously amplified PCR amplicons targeting the 16S rRNA gene.
What is the sequencing approach described?
The previously amplified amplicons will be barcoded (indexed) so multiple samples can be pooled and sequenced together using multiplex sequencing.
Which sequencing platform is specified?
The specified sequencing platform is the Illumina MiSeq.
What deliverables are expected from the funded work?
The expected deliverables are sequencing data and associated analysis outputs that the USGS project team can use to interpret microbial community patterns across sampling locations in Bighorn Lake.
What kind of analysis work is included?
The opportunity explicitly includes bioinformatics analysis associated with the sequencing effort, producing outputs to support interpretation of microbial community patterns.
Is this opportunity broadly scoped or narrowly scoped?
It is narrowly scoped. It is intended to fund sequencing and analysis of 16S rRNA gene amplicons derived from Bighorn Lake samples to support a broader USGS investigation into mercury methylation.
Who is eligible to apply?
Eligibility is listed as "Others," with a note that additional clarification would be provided in the full eligibility text in the original posting.
How many awards does the notice expect to make?
The notice anticipates a single award (Expected Awards: 1).
Is there an award ceiling (maximum funding amount) listed in the summary?
No. The award ceiling is shown as 0, which typically indicates that a ceiling is not specified in the summary fields and the funding amount would be defined elsewhere in the full announcement or through budget documentation/negotiation.
Does the opportunity state the exact award amount?
No exact funding amount is stated in the provided summary information.
What stage of lab work is implied by the description?
The description implies that sampling, DNA extraction, and PCR amplification have already occurred, and the funded work focuses on barcoding/indexing, Illumina MiSeq sequencing, and bioinformatics analysis of the existing 16S rRNA amplicons.
What kind of research output is this opportunity supporting within the larger USGS project?
It supports microbial community profiling data and analysis outputs that help the USGS team interpret differences in microbial communities across sites in the lake in relation to mercury methylation.
Browse more opportunities from the same category: Science and Technology and other Research and Development
Next opportunity: Small-Cell Lung Cancer (SCLC) Consortium: Therapeutic Development and Mechanisms of Resistance (U01)
Previous opportunity: Innovation Corps (I-Corps) at NIH Program for NIH and CDC Phase I Small Business Innovation Research (SBIR) and Small Business Technology Transfer (STTR) Grantees (Admin Supp )
USGrants.org Applicant Portal:
Are you interested in learning about about how to apply for this government funding opportunity? You can create a free applicant account and receive instant access to our applicant portal that many business owners like you have benefited from.
Apply for G16AS00012
[Watch] how do funding administrators access proposals?
Applicants also applied for:
Applicants who have applied for this opportunity (G16AS00012) also looked into and applied for these:
| Funding Opportunity |
|---|
| Funding Opportunity Announcement for the Navy and Marine Corps Science, Technology, Engineering Apply for N00014 16 R FO03 Funding Number: N00014 16 R FO03 Agency: DOD-ONR Category: Science and Technology and other Research and Development Funding Amount: $250,000 |
| FY16 Rail Safety Innovations Deserving Exploratory Analysis (IDEA) Co-operative Research Program Apply for FR RRD 16 001 Funding Number: FR RRD 16 001 Agency: DOT-FRA Category: Science and Technology and other Research and Development Funding Amount: $400,000 |
| D.2 Leading Edge Aeronautics Research for NASA Project (LEARN3) Apply for NNH15ZEA001N LEARN3 Funding Number: NNH15ZEA001N LEARN3 Agency: NASA-HQ Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Deployed Warfighter Protection Program FY 16 Call For Research Apply for DWFP 16 S 01 Funding Number: DWFP 16 S 01 Agency: DOD-AMC-ACCAPGD Category: Science and Technology and other Research and Development Funding Amount: $250,000 |
| Big Data Regional Innovation Hubs: Establishing Spokes to Advance Big Data Applications Apply for 16 510 Funding Number: 16 510 Agency: NSF Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| ROSES 2015: Ocean Biology and Biogeochemistry Apply for NNH15ZDA001N OBB Funding Number: NNH15ZDA001N OBB Agency: NASA-HQ Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Cooperative Ecosystem Studies Unit, Chesapeake Watershed CESU Apply for G16AS00014 Funding Number: G16AS00014 Agency: DOI-USGS1 Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Materials Engineering and Processing Apply for PD 16 8092 Funding Number: PD 16 8092 Agency: NSF Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Fluid Dynamics Research at the International Space Station to Benefit Life on Earth (NSF-ISS) Apply for 16 518 Funding Number: 16 518 Agency: NSF Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Deployed Warfighters Protection Program Apply for DWFP BAA 15 01 Funding Number: DWFP BAA 15 01 Agency: DOD-AMC-ACCAPGD Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| AFRL RD/RV University Cooperative Agreement Apply for BAA RVKV 2015 0003 Funding Number: BAA RVKV 2015 0003 Agency: DOD-AFRL Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Novel Instrumental Techniques for Basic Energy Research: Electrochemistry and Quantum Materials Apply for DE FOA 0001480 Funding Number: DE FOA 0001480 Agency: PAMS-SC Category: Science and Technology and other Research and Development Funding Amount: $750,000 |
| Manufacturing Machines and Equipment Apply for PD 16 1468 Funding Number: PD 16 1468 Agency: NSF Category: Science and Technology and other Research and Development Funding Amount: $300,000 |
| Complex Adaptive System Composition And Design Environment (CASCADE) Apply for DARPA BAA 16 11 Funding Number: DARPA BAA 16 11 Agency: DOD-DARPA-DSO Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| STARBASE Wright-Patt Apply for BAA AFRL RQKDA 2016 0001 Funding Number: BAA AFRL RQKDA 2016 0001 Agency: DOD-AFRL Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Plant Feedstock Genomics for Bioenergy: A Joint Research Apply for DE FOA 0001444 Funding Number: DE FOA 0001444 Agency: PAMS-SC Category: Science and Technology and other Research and Development Funding Amount: $400,000 |
| Undersea Technologies for Autonomous Detection and Communications Apply for N00014 16 R SN02 Funding Number: N00014 16 R SN02 Agency: DOD-ONR Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
| Algorithms in the Field Apply for 16 515 Funding Number: 16 515 Agency: NSF Category: Science and Technology and other Research and Development Funding Amount: $800,000 |
| Reverse Osmosis Concentrate Management through Halophyte Farming Apply for R16 NOA DO 006 Funding Number: R16 NOA DO 006 Agency: DOI-BOR Category: Science and Technology and other Research and Development Funding Amount: $75,000 |
| ROSES 2015: Early Career Fellowship Program Apply for NNH15ZDA001N ECF Funding Number: NNH15ZDA001N ECF Agency: NASA-HQ Category: Science and Technology and other Research and Development Funding Amount: Case Dependent |
Grant application guides and resources
It is always free to apply for government grants. However the process may be very complex depending on the funding opportunity you are applying for. Let us help you!
Apply for Grants

Premium leads for funding administrators, grant writers, and loan issuers
Thousands of people visit our website for their funding needs every day. When a user creates a grant proposal and files for submission, we pass the information on to funding administrators, grant writers, and government loan issuers.
If you manage government grant programs, provide grant writing services, or issue personal or government loans, we can help you reach your audience.
Subscribe to Leads
Request more information:
Would you like to learn more about this funding opportunity, similar opportunities to "G16AS00012", eligibility, application service, and/or application tips? Submit an inquiry below:
Don't forget to subscribe to our grant alerts mailing list to receive weekly alerts on new and updated grant funding opportunities like this one in your email.
