Opportunity Information: Apply for F12AS00259
Apply for F12AS00259
- The Fish and Wildlife Service in the environment sector is offering a public funding opportunity titled "Genetic Identification of Western and Yosemite Toads and Intergrades" and is now available to receive applicants.
- Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.650 Research Grants (Generic).
- This funding opportunity was created on Jun 26, 2012 and posted on Jun 26, 2012.
- Applicants must submit their applications by Jul 10, 2012. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
- The funding agency has allocated a total of $94,322.00 to eligible and selected applicants.
- Each selected applicant is eligible to receive up to $94,322.00 in funding.
- The number of recipients for this funding is limited to 1 candidate(s).
- Eligible applicants include: Public and State controlled institutions of higher education.
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Opportunity Summary:
The Fish and Wildlife Service funding opportunity titled "Genetic Identification of Western and Yosemite Toads and Intergrades" (Funding Opportunity Number F12AS00259) is a Notice of Intent to Award that was posted primarily to satisfy federal posting requirements. It is explicitly not an open solicitation and applicants are told not to apply. The announcement indicates a single-source award, meaning the agency already identified the intended recipient, and the public posting is procedural rather than competitive.
The project focus is an environmental and wildlife research effort aimed at improving genetic tools for distinguishing between the Yosemite toad (Anaxyrus canorus) and the western toad (Anaxyrus boreas), including situations where the two may overlap geographically and potentially hybridize. Yosemite toad populations have experienced substantial declines in recent decades, and the specific drivers are not fully understood. The notice highlights several suspected or implicated factors, including chytridiomycosis (a fungal disease caused by chytrid fungi), contaminants such as pesticide drift, and grazing impacts. Because conservation and management decisions depend heavily on accurate species identification and on understanding population structure, the agency is supporting development of a reliable identification method.
A core challenge described in the announcement is that field identification can be difficult, especially for larval and juvenile life stages and, in some cases, even for adults. Since the two species can occur in the same localities, misidentification can complicate monitoring, status assessments, and research on threats and recovery. To address this, the proposed work uses a RADseq (Restriction-site Associated DNA sequencing) next-generation sequencing approach. RADseq is intended to generate a large number of genetic markers across the genome, particularly single nucleotide polymorphisms (SNPs). With thousands of SNPs available, researchers can build clearer phylogenetic and population relationships among sampled individuals across a representative geographic range of both species.
Beyond constructing relationships and clarifying genetic structure, the notice emphasizes that the resulting dataset can be mined to identify species-diagnostic markers. The practical goal is to produce a dependable, rapid molecular assay that can be used to tell the species apart, and to recognize intergrades or hybrids where they occur. The announcement notes that these diagnostic markers would have broader scientific and management value, supporting work on species delimitation, interspecific hybridization, parentage and relatedness analyses, genetic diversity assessments, and other ecological and conservation questions that depend on accurate genetic identification.
Administratively, the opportunity is categorized as discretionary and uses an "Other" funding instrument type under the environment activity category. The agency expected a single award with a total estimated funding amount of $94,322, which is also listed as both the award ceiling and the award floor, indicating a fixed anticipated award size. There is no cost sharing or matching requirement. Eligibility is limited to public and state-controlled institutions of higher education, consistent with the research nature of the work. The posting date and creation date are June 26, 2012, with an original and current closing date of July 10, 2012, though the "do not apply" language makes clear it is not meant for general submission.
For reference and administrative questions, the notice provides a contact at the Fish and Wildlife Service: Valary Bloom (916) 414-6532, valarybloom@fws.gov. The listing also points to Grants.gov for access to the full announcement, again primarily for compliance and transparency rather than as an invitation for competitive applications.
FAQs: Genetic Identification of Western and Yosemite Toads and Intergrades (F12AS00259)
Is this a grant I can apply for?
No. This posting is explicitly a Notice of Intent to Award and is not an open solicitation. The announcement states that applicants should not apply.
Why was this opportunity posted publicly if it is not open for applications?
It was posted primarily to satisfy federal posting requirements. The public notice is described as procedural (for compliance and transparency) rather than competitive.
What does "Notice of Intent to Award" mean in this context?
It indicates the Fish and Wildlife Service already intends to make an award and is publishing the notice as part of required public reporting/posting practices, not to request competing proposals.
What does "single-source award" mean?
A single-source award means the agency has already identified the intended recipient, rather than selecting from multiple applicants through an open competition.
What is the title of the funding opportunity?
The title is "Genetic Identification of Western and Yosemite Toads and Intergrades."
What is the Funding Opportunity Number?
The Funding Opportunity Number is F12AS00259.
Which agency is associated with this opportunity?
The opportunity is associated with the U.S. Fish and Wildlife Service.
What is the project trying to accomplish?
The project aims to improve genetic tools for distinguishing between the Yosemite toad (Anaxyrus canorus) and the western toad (Anaxyrus boreas), including identifying intergrades or hybrids where the species overlap geographically.
Why is improved identification needed for these toads?
The notice explains that accurate identification is important for conservation and management decisions, monitoring, and understanding population structure. Field identification can be difficult, especially for larval and juvenile stages and sometimes even for adults, and misidentification can complicate assessments and research.
What conservation concern is mentioned for the Yosemite toad?
The notice states that Yosemite toad populations have experienced substantial declines in recent decades, and the specific drivers are not fully understood.
What threats or factors are suspected to be contributing to Yosemite toad declines?
The notice highlights suspected or implicated factors including chytridiomycosis (a fungal disease caused by chytrid fungi), contaminants such as pesticide drift, and grazing impacts.
What method is proposed to develop better genetic identification?
The proposed work uses RADseq (Restriction-site Associated DNA sequencing), a next-generation sequencing approach intended to generate many genetic markers across the genome.
What kind of genetic markers are expected from RADseq in this project?
The notice indicates RADseq is intended to produce a large number of markers, particularly single nucleotide polymorphisms (SNPs), potentially yielding thousands of SNPs for analysis.
How will the SNP dataset be used?
According to the notice, the SNP dataset will support clearer phylogenetic and population relationships among sampled individuals across a representative geographic range of both species, and it can be mined to identify species-diagnostic markers.
What is a "species-diagnostic marker" in the context of this notice?
In the notice, species-diagnostic markers are genetic markers that can reliably distinguish Yosemite toads from western toads and help identify intergrades or hybrids where they occur.
What is the practical end product described in the notice?
The practical goal is a dependable, rapid molecular assay that can be used to tell the species apart and recognize intergrades/hybrids.
Does the notice mention additional uses for the resulting genetic markers beyond basic identification?
Yes. The notice states the markers would have broader scientific and management value for topics including species delimitation, interspecific hybridization, parentage and relatedness analyses, genetic diversity assessments, and other ecological and conservation questions that rely on accurate genetic identification.
What is the activity category for this opportunity?
The notice places it under the environment activity category.
What type of funding opportunity is it described as?
It is categorized as discretionary.
What is the funding instrument type?
The funding instrument type is listed as "Other."
How many awards were expected?
The notice indicates the agency expected a single award.
What is the estimated total funding amount?
The total estimated funding amount is $94,322.
What are the award ceiling and award floor?
Both the award ceiling and the award floor are listed as $94,322, indicating a fixed anticipated award size in the posting.
Is cost sharing or matching required?
No. The notice states there is no cost sharing or matching requirement.
Who is eligible according to the posting?
Eligibility is limited to public and state-controlled institutions of higher education.
When was the notice posted and created?
The posting date and creation date are both June 26, 2012.
What is the closing date listed on the announcement?
The original and current closing date are both listed as July 10, 2012.
If there is a closing date, why does it still say not to apply?
The notice includes "do not apply" language and explains it is not an open solicitation. The presence of dates appears tied to the procedural nature of the posting rather than an invitation for competitive submissions.
Where is the full announcement referenced?
The listing points to Grants.gov for access to the full announcement, described as being for compliance and transparency rather than a competitive call for applications.
Who is the contact person for this notice?
The contact listed is Valary Bloom at the Fish and Wildlife Service.
What contact details are provided?
Phone: (916) 414-6532. Email: valarybloom@fws.gov.
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