Opportunity Information: Apply for L10AS00030
Apply for L10AS00030
- The Bureau of Land Management in the natural resources sector is offering a public funding opportunity titled "BLM WY Bluehead Sucker and Flannelmouth Sucker Genetic Analysis" and is now available to receive applicants.
- Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.231 Fish, Wildlife and Plant Conservation Resource Management.
- This funding opportunity was created on May 10, 2010 and posted on May 10, 2010.
- Applicants must submit their applications by May 25, 2010. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
- The funding agency has allocated a total of $15,543.00 to eligible and selected applicants.
- Each selected applicant is eligible to receive up to $15,543.00 in funding.
- The number of recipients for this funding is limited to 1 candidate(s).
- Eligible applicants include: Public and State controlled institutions of higher education.
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Opportunity Summary:
The BLM WY Bluehead Sucker and Flannelmouth Sucker Genetic Analysis opportunity (Funding Opportunity Number L10AS00030) was a Bureau of Land Management, Wyoming State Office discretionary grant issued as a cooperative agreement in the natural resources area under CFDA 15.231 (Fish, Wildlife and Plant Conservation Resource Management). The announcement was posted on May 10, 2010, with an application closing date of May 25, 2010, and it was later archived on June 30, 2010. BLM anticipated making a single award, and eligibility was limited to public and state-controlled institutions of higher education. The notice indicates BLM’s intent to award the agreement with the University of Wyoming, reflecting a targeted, partner-driven project rather than a broad open competition.
The project focuses on genetic analysis of sucker populations in Muddy Creek, with management emphasis on bluehead sucker and flannelmouth sucker conservation in the face of hybridization with non-native (and potentially other) lineages. The underlying management problem is “genome swamping,” where hybridization and repeated backcrossing can dilute or replace the genetic identity of native species over time. BLM sought genomic information that would directly support on-the-ground decisions, especially identifying and prioritizing removal of hybrids that are most likely to accelerate genetic introgression and compromise native populations. A second major management need is establishing clear, defensible criteria for selecting individuals that are as genetically “pure” as possible for conservation actions such as stocking, captive rearing, and other recovery or population-support efforts involving BLM sensitive fish species.
The technical approach described centers on generating next-generation sequencing data from Muddy Creek suckers using AFLP-based genetic techniques, with downstream analyses aimed at pinpointing the genomic architecture of hybridization. The University of Wyoming’s tasks were laid out in five main components: conducting next-generation DNA sequencing; identifying genetic regions that are important to ongoing hybridization between native and non-native fish; performing admixture mapping to link ancestry patterns to important phenotypic traits (observable characteristics potentially tied to fitness, habitat use, or identification); identifying candidate genes associated with those patterns; and translating the findings into information usable by managers and biologists working in native fisheries management. In practical terms, the project is meant to move from raw genetic data to actionable guidance, helping agencies distinguish between relatively unadmixed native individuals and those carrying introgressed segments that may be influential in continued hybridization dynamics.
The expected deliverables were twofold. First, the project would produce a manuscript summarizing key results of the hybridization “architecture” analysis, including comparisons or associations to established reference genomes such as zebrafish and stickleback. Those references function as well-annotated genomic baselines that can help interpret which regions or genes in suckers may be involved, even if sucker genomes themselves are less fully characterized. Second, the project would provide information on single nucleotide polymorphisms (SNPs), creating a set of genetic markers that could support additional analyses of catostomid (sucker family) hybridization across the broader Colorado River Basin. That SNP information is valuable because it can be reused for future monitoring, expanded population studies, or development of practical screening tools for managers.
The period of performance for the cooperative agreement was one year. Total estimated funding was fixed at $15,543, which was also the stated award ceiling and floor, with no cost-sharing or matching requirement. The grants.gov listing provided a point of contact for access issues: Ilze J. Karklins Powers, Grants Management Officer, Bureau of Land Management, reachable at 307-775-6293 or ipowers@blm.gov.
Frequently Asked Questions (FAQs)
What is the name of this funding opportunity?
The opportunity is titled "BLM WY Bluehead Sucker and Flannelmouth Sucker Genetic Analysis."
What is the Funding Opportunity Number (FON)?
The Funding Opportunity Number is L10AS00030.
Which agency offered this grant?
This was offered by the Bureau of Land Management (BLM), Wyoming State Office.
What type of funding instrument was used?
The opportunity was issued as a cooperative agreement, described as a discretionary grant.
What is the program area or topic?
The project falls under natural resources, specifically fish conservation and genetic analysis to support management actions.
What CFDA program is associated with this opportunity?
The opportunity is under CFDA 15.231, "Fish, Wildlife and Plant Conservation Resource Management."
When was the announcement posted?
The announcement was posted on May 10, 2010.
What was the application deadline?
The application closing date was May 25, 2010.
When was the opportunity archived?
It was later archived on June 30, 2010.
How many awards did BLM anticipate making?
BLM anticipated making a single award.
Who was eligible to apply?
Eligibility was limited to public and state-controlled institutions of higher education.
Was this a broadly competitive funding opportunity?
The notice indicates BLM's intent to award the agreement with the University of Wyoming, suggesting a targeted, partner-driven project rather than a broad open competition.
What species are the focus of the project?
The project focuses on bluehead sucker and flannelmouth sucker populations.
Where is the study area for the work described?
The work is focused on sucker populations in Muddy Creek.
What conservation problem is this project trying to address?
The management problem described is hybridization between native suckers and non-native (and potentially other) lineages, including the risk of "genome swamping," where repeated hybridization and backcrossing can dilute or replace the genetic identity of native species over time.
What is "genome swamping" in the context of this project?
In this opportunity, "genome swamping" refers to the process where hybridization and repeated backcrossing cause non-native genetic material to spread through a population and potentially overwhelm the native species' genetic identity.
How were the project results intended to be used by managers?
The project aimed to produce genomic information to support on-the-ground decisions, particularly helping identify and prioritize removal of hybrids that are most likely to accelerate genetic introgression and compromise native populations.
What additional management need does the project address besides hybrid removal?
A second major need is establishing clear, defensible criteria for selecting individuals that are as genetically "pure" as possible for conservation actions such as stocking, captive rearing, and other recovery or population-support efforts involving BLM sensitive fish species.
What genetic methods or technologies were highlighted?
The approach described centers on generating next-generation sequencing data from Muddy Creek suckers using AFLP-based genetic techniques, followed by analyses intended to understand the genomic architecture of hybridization.
What were the main tasks assigned to the University of Wyoming?
The work was described in five main components: (1) conducting next-generation DNA sequencing; (2) identifying genetic regions important to ongoing hybridization between native and non-native fish; (3) performing admixture mapping to link ancestry patterns to important phenotypic traits; (4) identifying candidate genes associated with those patterns; and (5) translating findings into information usable by managers and biologists working in native fisheries management.
What is admixture mapping as described in this project?
Admixture mapping, in the context provided, is an analysis intended to connect ancestry patterns in hybridizing populations to important phenotypic traits (observable characteristics) that may relate to fitness, habitat use, or identification.
What kind of deliverables were expected from the project?
Two deliverables were described: (1) a manuscript summarizing key results of the hybridization "architecture" analysis, including comparisons or associations to reference genomes such as zebrafish and stickleback; and (2) information on single nucleotide polymorphisms (SNPs) to create genetic markers useful for additional catostomid hybridization analyses across the broader Colorado River Basin.
Why were zebrafish and stickleback mentioned?
They were referenced as established, well-annotated reference genomes that could help interpret which genomic regions or genes in suckers may be involved in hybridization, even if sucker genomes are less fully characterized.
What are SNPs and why were they important for this opportunity?
The opportunity called for SNP (single nucleotide polymorphism) information to create genetic markers that could support further hybridization analyses in catostomid fishes across the Colorado River Basin, enabling future monitoring, expanded population studies, or development of screening tools for managers.
What was the period of performance?
The cooperative agreement period of performance was one year.
How much funding was available?
Total estimated funding was $15,543.
What were the award floor and ceiling?
The stated award ceiling was $15,543 and the award floor was also $15,543.
Was cost sharing or matching required?
No cost-sharing or matching requirement was stated.
Who was the point of contact for access issues related to the listing?
The point of contact listed for access issues was Ilze J. Karklins Powers, Grants Management Officer, Bureau of Land Management.
How can the listed contact be reached?
Phone: 307-775-6293. Email: ipowers@blm.gov.
What was the intended practical outcome of the genetic work?
In practical terms, the project was intended to move from raw genetic data to actionable guidance that helps agencies distinguish between relatively unadmixed native individuals and those carrying introgressed genetic segments that may influence ongoing hybridization dynamics.
Was the project described as supporting work beyond Muddy Creek?
Yes. One deliverable was SNP marker information intended to support additional analyses of catostomid hybridization across the broader Colorado River Basin.
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