Opportunity Information: Apply for F14AS00107
Apply for F14AS00107
- The Fish and Wildlife Service in the natural resources science and technology and other research and development sector is offering a public funding opportunity titled "Discovery, validation, and genotyping of single nucleotide polymorphisms in Pacific walrus (Odobenus rosmarus divergens)" and is now available to receive applicants.
- Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.608 Fish and Wildlife Management Assistance.
- This funding opportunity was created on Mar 5, 2014 and posted on Mar 5, 2014.
- Applicants must submit their applications by Mar 19, 2014. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
- The funding agency has allocated a total of $23,000.00 to eligible and selected applicants.
- Each selected applicant is eligible to receive up to $23,000.00 in funding.
- The number of recipients for this funding is limited to 1 candidate(s).
- Eligible applicants include: Unrestricted (i.e., open to any type of entity above), subject to any clarification in text field entitled Additional Information on Eligibility.
- All potential applicants are eligible that meet the following requirements 1. laboratory capacity to conduct next generation sequencing using a genome reduction technique such as restriction site associated DNA (RAD) tag sequencing 2. The computational capacity and ability to screen and analyze RAD tag sequence data to provide the Service with sufficient sequence for each polymorphism to develop SNP assays for the Life Technologies Quant Studio PCR system.
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Opportunity Summary:
The U.S. Fish and Wildlife Service (FWS) grant opportunity F14AS00107 supports a focused genetics project on Pacific walrus (Odobenus rosmarus divergens) from Alaska. It is a discretionary award offered as a cooperative agreement, meaning the work is expected to be carried out in coordination with the agency rather than as a completely hands-off research grant. The project’s core deliverable is a large, standardized set of genetic markers that FWS can apply directly to management needs, especially population assessment and sex identification from field samples.
The scientific scope centers on discovering, validating, and genotyping roughly 10,000 single nucleotide polymorphisms (SNPs) using samples from 192 individual Pacific walruses. A specific emphasis is placed on including around 100 SNP loci with high minor allele frequency (MAF of at least about 0.30), since higher-frequency variants tend to be more informative for individual identification, assignment, and mark-recapture style analyses. Alongside this broad SNP development, the project also calls for a thorough bioinformatics effort aimed at identifying sex-specific SNPs, which can provide a genetic method to determine the sex of sampled walruses, including cases where field-based sexing is uncertain or impossible.
FWS intends to use the SNP panel to meet two applied objectives. First, the markers will support estimation of walrus population abundance using a genetic mark-recapture approach. In practice, this relies on genotypes functioning like “genetic tags†so that the same individual can be recognized across multiple sampling events, enabling abundance estimation without physically marking animals. Second, the work will support genetic determination of sex in sampled animals, which is important for demographic analyses, interpreting survey data, and improving the usefulness of samples collected under challenging field conditions.
Eligibility is broadly open (“unrestrictedâ€) to any applicant type, but applicants must be able to meet two practical capacity requirements. They must have laboratory capability to conduct next-generation sequencing using a genome reduction approach, explicitly mentioning methods such as restriction site associated DNA (RAD) tag sequencing. They must also have the computational resources and expertise to process, screen, and analyze RAD tag data and then provide FWS with sufficient sequence context for each polymorphism so that SNP assays can be designed for the Life Technologies Quant Studio PCR system. In other words, the outcome is not only discovery of SNPs, but also delivery of assay-ready information compatible with a specific genotyping platform used by the Service.
The funding profile is small and tightly defined: one expected award with a total of $23,000, which is also the award ceiling and floor. There is no cost sharing or matching requirement. The opportunity was posted March 5, 2014, and closed March 19, 2014, with an archive date of May 5, 2014. It is associated with CFDA 15.608 (Fish and Wildlife Management Assistance) and falls under natural resources science and technology and other research and development.
For additional program context, the announcement points to an FWS Alaska genetics webpage, and the listed contact for access issues or questions is Katherine Smiley at the FWS (907-786-3412; KatherineSmiley@fws.gov).
Frequently Asked Questions (FAQ)
What is the grant opportunity F14AS00107?
F14AS00107 is a U.S. Fish and Wildlife Service (FWS) grant opportunity supporting a focused genetics project on Pacific walrus (Odobenus rosmarus divergens) from Alaska. The project is designed to produce genetic tools that FWS can apply directly to management needs, especially population assessment and sex identification from field samples.
Which agency is offering this opportunity?
The opportunity is offered by the U.S. Fish and Wildlife Service (FWS).
What type of award is it?
It is a discretionary award offered as a cooperative agreement. That means the work is expected to be carried out in coordination with FWS rather than as a fully hands-off research grant.
What species and population does the project focus on?
The project focuses on Pacific walrus (Odobenus rosmarus divergens) from Alaska.
What is the main deliverable expected from the project?
The core deliverable is a large, standardized set of genetic markers that FWS can use for management applications, with emphasis on population assessment and genetic sex identification from field-collected samples.
What genetic markers are being developed?
The scientific scope centers on discovering, validating, and genotyping roughly 10,000 single nucleotide polymorphisms (SNPs) using samples from 192 individual Pacific walruses.
How many samples (individual walruses) are included in the project scope?
The scope specifies samples from 192 individual Pacific walruses.
Is there a requirement for high-frequency SNPs (high MAF loci)?
Yes. A specific emphasis is placed on including around 100 SNP loci with high minor allele frequency (MAF of at least about 0.30). Higher-frequency variants are intended to be more informative for individual identification, assignment, and mark-recapture style analyses.
What bioinformatics work is required?
The project calls for a thorough bioinformatics effort to identify sex-specific SNPs. These sex-linked markers are intended to provide a genetic method for determining the sex of sampled walruses, including when field-based sexing is uncertain or not possible.
How does FWS plan to use the SNP panel for population assessment?
FWS intends to use the markers to estimate walrus population abundance using a genetic mark-recapture approach. In this method, genotypes function like genetic tags so the same individual can be recognized across multiple sampling events without physically marking animals.
How does the project support sex identification?
The work is intended to enable genetic determination of sex in sampled walruses. This supports demographic analyses, interpretation of survey data, and improves the usefulness of samples collected under difficult field conditions where sex may be hard to determine visually.
Who is eligible to apply?
Eligibility is listed as unrestricted, meaning any applicant type may apply, as long as the applicant can meet the project capacity requirements described in the announcement.
What laboratory capabilities are required to be a viable applicant?
Applicants must have laboratory capability to conduct next-generation sequencing using a genome reduction approach. The announcement explicitly references methods such as restriction site associated DNA (RAD) tag sequencing.
What computational and data analysis capabilities are required?
Applicants must have computational resources and expertise to process, screen, and analyze RAD tag data. They must also be able to provide FWS with enough sequence context for each polymorphism so SNP assays can be designed.
Is a specific genotyping platform required for assay design compatibility?
Yes. The project requires delivery of assay-ready information compatible with the Life Technologies Quant Studio PCR system used by the Service. This means the outcome is not only SNP discovery, but also providing sufficient sequence context to support assay design for that platform.
How much funding is available?
The total expected award amount is $23,000.
What are the award floor and ceiling?
The award floor is $23,000 and the award ceiling is also $23,000, indicating a single, fixed funding amount.
How many awards does FWS expect to make?
The funding profile indicates one expected award.
Is cost sharing or a match required?
No. The opportunity states there is no cost sharing or matching requirement.
When was the opportunity posted and when did it close?
The opportunity was posted on March 5, 2014, and closed on March 19, 2014.
What is the archive date for this opportunity?
The archive date listed is May 5, 2014.
What CFDA program is associated with this opportunity?
The opportunity is associated with CFDA 15.608, Fish and Wildlife Management Assistance.
What general research area does the opportunity fall under?
It falls under natural resources science and technology and other research and development, as described in the announcement.
Where can applicants find additional program context mentioned in the announcement?
The announcement points to an FWS Alaska genetics webpage for additional program context.
Who is the listed contact for questions or access issues?
The listed contact is Katherine Smiley at FWS. Phone: (907) 786-3412. Email: KatherineSmiley@fws.gov.
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