Opportunity Information: Apply for F15AS00410
Apply for F15AS00410
- The Fish and Wildlife Service in the environment natural resources sector is offering a public funding opportunity titled "eDNA at Moapa Valley National Wildlife Refuge" and is now available to receive applicants.
- Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.608 Fish and Wildlife Management Assistance.
- This funding opportunity was created on Jul 31, 2015 and posted on Jul 31, 2015.
- Applicants must submit their applications by Aug 5, 2015. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
- The funding agency has allocated a total of $47,000.00 to eligible and selected applicants.
- Each selected applicant is eligible to receive up to $47,000.00 in funding.
- The number of recipients for this funding is limited to 1 candidate(s).
- Eligible applicants include: Unrestricted (i.e., open to any type of entity above), subject to any clarification in text field entitled Additional Information on Eligibility.
- THE RECIPIENT HAS BEEN SELECTED. SEE ATTACHED NOTICE OF INTENT TO AWARD.
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Opportunity Summary:
The grant opportunity titled "eDNA at Moapa Valley National Wildlife Refuge" is a U.S. Fish and Wildlife Service cooperative agreement focused on building an environmental DNA (eDNA) monitoring protocol to improve early detection of invasive aquatic species in and around Moapa Valley National Wildlife Refuge, the Warm Springs Natural Area, and nearby connected waters. The central goal is to create a practical, field-ready eDNA tracking approach that can identify the presence of three specific invasive species at very low levels before they become widespread: blue tilapia (Oreochromis aureus), red shiner (Cyprinella lutrensis), and red swamp crayfish (Procambarus clarkii). By detecting these organisms early, managers can respond quickly and reduce ecological damage, especially in sensitive desert spring and refuge habitats where invasives can rapidly alter food webs, water quality, and native species survival.
The project is designed to align directly with on-the-ground invasive species control actions. The eDNA work is intended to be coordinated with planned or ongoing efforts such as removing fish barriers and conducting chemical eradication treatments. In other words, the monitoring is not being developed in isolation; it is meant to support real management decisions by confirming whether invasive species are present, tracking whether removals are working, and providing an early warning system for re-invasion after barriers are modified or treatments occur. This integration makes the protocol more useful than a purely academic study, since it can be timed and structured around the points when managers most need reliable detection information.
A key feature of the opportunity is that it builds on existing methods rather than starting from scratch. The notice explains that an eDNA protocol previously developed for crayfish at Ash Meadows National Wildlife Refuge will be revised and expanded to cover two additional invasive species (blue tilapia and red shiner) while still addressing red swamp crayfish. This implies the work will likely include adapting sampling designs, laboratory assays (such as species-specific primers/probes), and analysis workflows so that the resulting protocol can be applied consistently across the refuge and surrounding waters. The deliverable is essentially a refined, multi-species eDNA detection protocol that can be used to support routine surveillance and rapid response.
From an administrative standpoint, this was a discretionary funding opportunity under the Environment and Natural Resources activity category, offered as a cooperative agreement through the Fish and Wildlife Service. The CFDA program number listed is 15.608 (Fish and Wildlife Management Assistance). The estimated total funding was $47,000, with an award ceiling of $47,000 and an award floor of $100, and there was no cost-sharing or matching requirement. Only one award was expected. The posting date was July 31, 2015, with an original and final closing date of August 5, 2015, and an archive date of August 6, 2015, reflecting a very short application window typical of situations where an intended recipient has already been identified.
Eligibility was listed as unrestricted in general terms, but the announcement explicitly states that the recipient had already been selected, with a notice of intent to award referenced in the opportunity materials. That means this posting functioned primarily as a formal public notice rather than an open competition. The agency contact provided for access issues or questions was Joanne Hammaren (702-515-5454, joannehammaren@fws.gov). Overall, the opportunity represents a targeted investment in early detection tools for invasive aquatic species, intended to strengthen refuge management capacity and support implementation of the relevant recovery planning efforts and the Comprehensive Conservation Plan for the Desert National Wildlife Refuge Complex.
Frequently Asked Questions (FAQs): eDNA at Moapa Valley National Wildlife Refuge
What is the "eDNA at Moapa Valley National Wildlife Refuge" grant opportunity?
This opportunity was a U.S. Fish and Wildlife Service (USFWS) cooperative agreement focused on building an environmental DNA (eDNA) monitoring protocol to improve early detection of invasive aquatic species in and around Moapa Valley National Wildlife Refuge, the Warm Springs Natural Area, and nearby connected waters.
What is the main purpose of this project?
The central goal is to create a practical, field-ready eDNA tracking approach that can identify the presence of specific invasive aquatic species at very low levels, before they become widespread. The intent is to give managers an early warning tool so they can respond quickly and reduce ecological damage in sensitive desert spring and refuge habitats.
Which invasive species are targeted for eDNA detection under this project?
The protocol is intended to detect three invasive species:
- Blue tilapia (Oreochromis aureus)
- Red shiner (Cyprinella lutrensis)
- Red swamp crayfish (Procambarus clarkii)
Why is early detection of these species important for the refuge and nearby waters?
The opportunity materials emphasize that invasive species can rapidly alter desert spring and refuge ecosystems by changing food webs, affecting water quality, and reducing native species survival. Detecting invasives early can help managers act before populations expand and impacts become harder to reverse.
What geographic areas does the project focus on?
The project is centered on Moapa Valley National Wildlife Refuge, the Warm Springs Natural Area, and nearby connected waters in the surrounding area.
What does "environmental DNA (eDNA)" mean in the context of this project?
In this context, eDNA refers to monitoring approaches that detect the presence of target species using DNA found in the environment (for example, in water). The aim is a monitoring protocol that can detect targeted invasive species even when they are present at very low levels.
Is this project meant to be research-only, or will it support real management decisions?
The notice indicates it is designed to align directly with on-the-ground invasive species control actions. The monitoring protocol is meant to support management decisions by confirming presence of invasive species, tracking whether removals are working, and providing early warning for re-invasion.
How will the eDNA monitoring be coordinated with invasive species control actions?
The opportunity describes coordination with planned or ongoing actions such as removing fish barriers and conducting chemical eradication treatments. The protocol is intended to be timed and structured around moments when managers most need reliable detection information, including before and after management actions.
What is the expected deliverable from this cooperative agreement?
The key deliverable is a refined, multi-species eDNA detection protocol that can be applied consistently across the refuge and surrounding connected waters to support routine surveillance and rapid response.
Is the project creating a brand-new eDNA method from scratch?
No. A specific feature of the opportunity is that it builds on existing methods. The notice explains that an eDNA protocol previously developed for crayfish at Ash Meadows National Wildlife Refuge would be revised and expanded to cover blue tilapia and red shiner while still addressing red swamp crayfish.
What existing eDNA work is being leveraged?
The announcement states that a crayfish eDNA protocol developed for Ash Meadows National Wildlife Refuge is the starting point, and it would be updated and expanded to include additional species relevant to Moapa Valley National Wildlife Refuge and connected waters.
What kinds of technical components might be updated in the protocol?
The information provided suggests the work would likely involve adapting sampling designs, laboratory assays (including species-specific primers/probes), and analysis workflows so the final protocol can be used consistently for multiple target species.
What federal agency is offering this funding?
The funding opportunity was offered by the U.S. Fish and Wildlife Service as a cooperative agreement.
What type of funding mechanism is this?
It was issued as a cooperative agreement, under a discretionary funding opportunity.
What is the activity category listed for this grant?
The activity category was Environment and Natural Resources.
What is the CFDA program number associated with this opportunity?
The CFDA program number listed is 15.608, Fish and Wildlife Management Assistance.
How much funding was available?
The estimated total funding was $47,000.
What were the award ceiling and award floor?
The award ceiling was $47,000 and the award floor was $100.
Was cost sharing or matching required?
No. The notice states there was no cost-sharing or matching requirement.
How many awards were expected to be made?
Only one award was expected.
When was the opportunity posted?
The posting date was July 31, 2015.
What were the application open and close dates?
The original and final closing date was August 5, 2015, indicating a very short application window.
When was the opportunity archived?
The archive date was August 6, 2015.
Was this an open, competitive funding opportunity?
Although eligibility was listed as unrestricted in general terms, the announcement explicitly states that the recipient had already been selected and references a notice of intent to award. That indicates this posting functioned primarily as a formal public notice rather than an open competition.
If eligibility was listed as unrestricted, why was the application window so short?
The materials describe a short window that is typical of postings where an intended recipient has already been identified. In this case, the opportunity states the recipient had already been selected via a notice of intent to award.
Who should be contacted with questions or access issues about the opportunity?
The agency contact listed is Joanne Hammaren. Phone: 702-515-5454. Email: joannehammaren@fws.gov.
How does this work connect to broader refuge planning or recovery efforts?
The opportunity is described as a targeted investment intended to strengthen refuge management capacity and support implementation of relevant recovery planning efforts and the Comprehensive Conservation Plan for the Desert National Wildlife Refuge Complex.
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