Opportunity Information: Apply for F15AS00045

  • The Fish and Wildlife Service in the other (see text field entitled explanation of other category of funding activity for clarification) sector is offering a public funding opportunity titled "Identifying genetic groups in the Eastern populaion of the toad species group" and is now available to receive applicants.
  • Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.660 Endangered Species Candidate Conservation Action Funds.
  • This funding opportunity was created on Dec 9, 2014 and posted on Dec 9, 2014.
  • Applicants must submit their applications by Dec 16, 2014 This is a notice of intent to award a CESU Cooperative agreement to Florida Gulf Coast University under the South Florida Caribbean CESU. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
  • The funding agency has allocated a total of $70,000.00 to eligible and selected applicants.
  • Each selected applicant is eligible to receive up to $70,000.00 in funding.
  • The number of recipients for this funding is limited to 1 candidate(s).
  • Eligible applicants include: Others (see text field entitled Additional Information on Eligibility for clarification).
  • This is a notice of intent to award a CESU Cooperative agreement to Florida Gulf Coast University under the South Florida Caribbean CESU
Apply for F15AS00045

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Opportunity Summary:

This funding opportunity (Funding Opportunity Number F15AS00045) is a U.S. Fish and Wildlife Service cooperative agreement focused on clarifying genetic structure within the boreal toad species group, with particular emphasis on identifying and mapping distinct genetic groups in the Eastern Population. The core purpose is to generate and analyze nuclear DNA sequence information to determine how many genetically distinct groups exist, how they relate to previously identified mitochondrial DNA (mtDNA) lineages, and where the boundaries between major groups likely fall across the landscape. The results are meant to support conservation planning under the Endangered Species program and Candidate Conservation Action Funds (CFDA 15.660), especially where management decisions depend on whether populations represent meaningful evolutionary or conservation units.

The work is organized around three linked sampling and analysis phases. First, the project calls for obtaining nuclear gene sequence data from roughly 25 to 40 samples that collectively represent all known species and the major divergent mtDNA lineages across the entire geographic range of the boreal toad species group. This broad-range dataset is intended to provide a species-group-wide genetic context and help distinguish deep lineages from more local population structure. Second, the project targets the Eastern Population specifically by generating sequence data from the most informative nuclear genes for about 100 samples that are already mostly in hand. These samples are designed to represent the full known distribution of the Eastern Population and also include toads from immediately surrounding areas in Wyoming, Idaho, Utah, and Nevada, which is important for evaluating transitions and potential gene flow at the edges of the Eastern Population. Third, the opportunity anticipates an additional round of collection and analysis from an estimated 100 samples to sharpen conclusions from the first two phases. This final phase is explicitly described as a refinement step to better delineate geographic boundaries of the major genetic groups, confirm the distinctiveness of specific groups of interest (with examples including the SRM population and southern Utah), and verify any newly detected groups that emerge from the earlier analyses.

Administratively, this is a discretionary opportunity using a cooperative agreement as the funding instrument, and it is described as a notice of intent to award through the Cooperative Ecosystem Studies Units (CESU) network. The notice specifies an intended recipient: Florida Gulf Coast University under the South Florida Caribbean CESU. The opportunity indicates one expected award with a total estimated funding amount of $70,000, and both the award ceiling and floor are listed as $70,000, signaling a fixed anticipated award size rather than a range. No cost sharing or matching requirement is listed. The opportunity was posted and created on December 9, 2014, with an original and current closing date of December 16, 2014, and an archive date of January 9, 2015.

For applicants or parties needing assistance accessing the announcement, the listed agency contact is Terry Ireland (U.S. Fish and Wildlife Service) at 970-628-7188 and terryireland@fws.gov.

Frequently Asked Questions (FAQs)

What is the funding opportunity number for this grant?

The funding opportunity number is F15AS00045.

Which federal agency is offering this opportunity?

This opportunity is offered by the U.S. Fish and Wildlife Service (U.S. Department of the Interior).

What type of award is being used?

The funding instrument is a cooperative agreement.

Is this a discretionary funding opportunity?

Yes. The opportunity is described as discretionary.

What is the main purpose of this cooperative agreement?

The purpose is to clarify genetic structure within the boreal toad species group, with a particular emphasis on identifying and mapping distinct genetic groups in the Eastern Population. The project focuses on generating and analyzing nuclear DNA sequence information to determine how many genetically distinct groups exist, how those groups relate to previously identified mitochondrial DNA (mtDNA) lineages, and where major group boundaries likely occur across the landscape.

Why is the project focused on nuclear DNA if mtDNA lineages already exist?

The opportunity specifically calls for nuclear DNA sequence data to evaluate genetic structure and to connect (or distinguish) nuclear genetic groupings relative to previously identified mtDNA lineages. The stated goal is to determine how many distinct genetic groups exist and how they relate to mtDNA patterns.

How will the results be used?

The results are intended to support conservation planning under the Endangered Species program and Candidate Conservation Action Funds (CFDA 15.660), particularly where management decisions depend on whether populations represent meaningful evolutionary or conservation units.

What species or species group does the work address?

The work addresses the boreal toad species group, with special emphasis on the Eastern Population.

What are the main technical goals described in the opportunity?

The opportunity describes three core technical goals: (1) generating nuclear gene sequence data to provide species-group-wide context, (2) focusing on the Eastern Population to analyze the most informative nuclear genes across its distribution and nearby areas, and (3) refining conclusions with additional sampling to better delineate boundaries and confirm distinctiveness of specific groups.

How is the scientific work organized?

The work is organized into three linked sampling and analysis phases.

What is included in Phase 1?

Phase 1 calls for obtaining nuclear gene sequence data from roughly 25 to 40 samples representing all known species and the major divergent mtDNA lineages across the entire geographic range of the boreal toad species group. This broad dataset is intended to provide species-group-wide genetic context and help distinguish deep lineages from more local population structure.

What is included in Phase 2?

Phase 2 targets the Eastern Population by generating sequence data from the most informative nuclear genes for about 100 samples that are already mostly in hand. These samples are designed to represent the full known distribution of the Eastern Population and also include nearby toads from Wyoming, Idaho, Utah, and Nevada to evaluate transitions and potential gene flow at the population edges.

What is included in Phase 3?

Phase 3 anticipates additional collection and analysis from an estimated 100 samples. This is described as a refinement step intended to sharpen conclusions from the first two phases by better delineating geographic boundaries of major genetic groups, confirming the distinctiveness of specific groups of interest (examples given include the SRM population and southern Utah), and verifying any newly detected groups that emerge from earlier analyses.

How many samples are expected to be used across all phases?

Based on the described estimates, the project anticipates roughly 25 to 40 samples in Phase 1, about 100 samples in Phase 2, and an estimated additional 100 samples in Phase 3 (for a total on the order of approximately 225 to 240 samples, depending on the Phase 1 sample count).

Which geographic areas are specifically mentioned for the Eastern Population work?

The opportunity notes that Phase 2 sampling is designed to cover the full known distribution of the Eastern Population and includes surrounding areas in Wyoming, Idaho, Utah, and Nevada to help evaluate transitions and potential gene flow at the edges.

Are any specific groups or locations called out as priorities for confirmation?

Yes. In Phase 3, examples of groups of interest that may need confirmation include the SRM population and southern Utah, along with any newly detected groups identified during earlier analyses.

How many awards does the agency expect to make under this announcement?

The opportunity indicates one expected award.

What is the total estimated funding amount?

The total estimated funding amount is $70,000.

Is the funding amount a range or a fixed anticipated award size?

The award ceiling and award floor are both listed as $70,000, which indicates a fixed anticipated award size rather than a funding range.

Is cost sharing or matching required?

No cost sharing or matching requirement is listed in the opportunity.

What is the program or CFDA number associated with this work?

The opportunity references Candidate Conservation Action Funds under CFDA 15.660.

Is this opportunity connected to the CESU network?

Yes. It is described as a notice of intent to award through the Cooperative Ecosystem Studies Units (CESU) network.

Who is the intended recipient named in the notice?

The notice specifies an intended recipient: Florida Gulf Coast University under the South Florida Caribbean CESU.

When was the opportunity posted?

The opportunity was posted (and created) on December 9, 2014.

What was the closing date for this opportunity?

The original and current closing date is listed as December 16, 2014.

When was the opportunity archived?

The archive date is January 9, 2015.

Who should be contacted for help accessing the announcement or for questions?

The listed agency contact is Terry Ireland (U.S. Fish and Wildlife Service).

What contact information is provided for the agency point of contact?

Phone: 970-628-7188. Email: terryireland@fws.gov.

Does the announcement indicate whether samples for Phase 2 are already available?

Yes. It states that the Phase 2 dataset includes about 100 samples that are already mostly in hand.

What is the overarching conservation decision need behind the genetic work?

The opportunity explains that conservation and management decisions may depend on whether populations represent meaningful evolutionary or conservation units, and the genetic structure and boundary mapping are intended to inform that planning.

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